Journal: Pain Physician

Article Title: Evaluation of the Effect of Duration on the Efficacy of Pulsed Radiofrequency in an Animal Model of Neuropathic Pain

doi: 10.36076/ppj.2018.2.191

Figure Lengend Snippet: Fig. 1. Immunopositive nerve fibers for TNF-α of CCI rats after scarification.

Article Snippet: Paraformaldehyde-fixed paraffin-embedded sciatic nerve sections (5 mm thickness) were incubated with antibodies to TNF-α (MP6-XT22, Novus Biologicals, Littleton, CO) and IL-6 (20F3, Thermo Fisher Scientific, Waltham, MA).

Techniques:

Journal: STAR Protocols

Article Title: Protocol to isolate immune cells from mouse pancreatic lymph nodes and whole pancreas for mass cytometric analyses

doi: 10.1016/j.xpro.2022.101938

Figure Lengend Snippet:

Article Snippet: Anti-mouse TNFα, Clone MP6-XT22, 1:50 dilution , Fluidigm , Cat#3162002C, Label 162Dy.

Techniques: Recombinant, Staining, Red Blood Cell Lysis, Software, Cytometry

Journal: Experimental cell research

Article Title: Using phage-assisted continuous evolution (PACE) to evolve human PD1.

doi: 10.1016/j.yexcr.2020.112244

Figure Lengend Snippet: Fig. 3. Summarization of PD1 variants that bind PDL1. (A) Under high levels of mutagenesis (MP6) during the 288 h of PACE. The Red line indicates the phage titer of each time point. And the grey line indicates the flow velocity of system. (B) During 288 h of evolution, “lagoon” samples were sequenced using the Sanger method, and 11 different preponderant oligotypes were identified which contained 9 amino acid sites. (C) Among these 11 oligotypes, 9 amino acids exhibited diverse retention from 9 times of T98L to 3 times G18A. (D) The summarization of 11 oligotypes is shown on a time axis.

Article Snippet: SP098, pAB107a, MP6 plasmid, S2060 and S2208 were obtained from Addgene.

Techniques: Mutagenesis

Journal: Bio-protocol

Article Title: Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices

doi: 10.21769/BioProtoc.3387

Figure Lengend Snippet: A. The cables connecting the different components are color-coded for easy assembly of the circuit; a step-by-step outline is provided in Figures 4-14. B. Color-coded scheme of the connections on the Molex connector. The connections labeled -P1, +P1, +P2, and -P2 should be connected to the four output pins of the OEMs as dictated by the colors. For fast and secure fastening/unfastening of the micopump assemblies, use a Pi Cobbler Breakout and associated cable. C. Photograph of an mp6 micropump and Molex connector from B. Note that only one side of the micropump has markings (the company logo and micropump serial number). Slide the micropump through the slot, in the orientation shown, and close the Molex component to secure the connection.

Article Snippet: The system employs the mp6 micropump, a piezoelectric diaphragm pump developed by Bartels Mikrotechnik GmbH (Dortmund, Germany) for the transport of liquids or gases at varying flow rates and/or pressures controlled by an external electronics circuit.

Techniques: Labeling

Journal: Bio-protocol

Article Title: Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices

doi: 10.21769/BioProtoc.3387

Figure Lengend Snippet: A and B. Two mp6 micropumps are stacked in parallel connection, which causes their volume flows to summate. Each such “sandwich” array consists of three custom-made plexiglass panels, one of which is threaded so that screws can hold the assembly together. Cork pads cut to size allow for precise contact of different surfaces. C. Two assemblies shown in (A) and (B) stacked on top of each other, constitute the pump array that controls the suction. Notice the y connectors and mp-cv valves. D. The 26-pin Assembled Pi Cobbler Breakout Board is connected to 26-pin shield stacking headers with longer pins, so as to allow enough clearance for two of the mp6-OEM controllers that are attached directly adjacent to it. E. Cable that connects six micropumps to the Pi Cobbler Breakout Board. F. Mini-Faraday cages made of copper wire mesh to allow grounding the noise from the pumps.

Article Snippet: The system employs the mp6 micropump, a piezoelectric diaphragm pump developed by Bartels Mikrotechnik GmbH (Dortmund, Germany) for the transport of liquids or gases at varying flow rates and/or pressures controlled by an external electronics circuit.

Techniques:

Journal: Bio-protocol

Article Title: Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices

doi: 10.21769/BioProtoc.3387

Figure Lengend Snippet: The program allows turning all six of the mp6 micropumps on simultaneously when prompted by one of the digital outputs of the ADC, which is in turn controlled by the “Slow0 Perfusion Change” function of the winLTP program. By changing the “delay” value (highlighted in yellow) the slices can be perfused for variable intervals. In this particular example the pumps will remain on for 1800000 ms (i.e., 30 min).

Article Snippet: The system employs the mp6 micropump, a piezoelectric diaphragm pump developed by Bartels Mikrotechnik GmbH (Dortmund, Germany) for the transport of liquids or gases at varying flow rates and/or pressures controlled by an external electronics circuit.

Techniques:

Journal: Bio-protocol

Article Title: Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices

doi: 10.21769/BioProtoc.3387

Figure Lengend Snippet: The program allows changing the frequency of the signal generated by the mp6-OEM from the default value, to reduce the mp6 micropump flow volume from its maximum value (5.5 ml/min). The frequency of the OEM signal is altered by changing the value of “int32_t clockfrequency” (highlighted in yellow). The current value “int32_t clockfrequency = 75” yields a flow rate of approximately 2.5 ml/min (see Figure 20).

Article Snippet: The system employs the mp6 micropump, a piezoelectric diaphragm pump developed by Bartels Mikrotechnik GmbH (Dortmund, Germany) for the transport of liquids or gases at varying flow rates and/or pressures controlled by an external electronics circuit.

Techniques: Generated